2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Standaardstowwe wat in die kalibrasiekurwe van relatiewe molekulêre massaverspreiding gebruik word: insulien, mikopeptiede, glisien-glisien-tirosien-arginien, glisien-glisien-glisien
3 Instrument en toerusting
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Oor die algemeen is die verhouding van aminosure in Sustar se produkte hoër as dié in Zinpro se produkte.
Deel 8 Gevolge van gebruik
Effekte van verskillende bronne van spoorelemente op die produksieprestasie en eiergehalte van lêhenne in die laat lêperiode
Produksieproses
Gerigte chelasietegnologie
Skuif-emulsifikasietegnologie
Drukspuit- en droogtegnologie
Verkoelings- en ontvochtigingstegnologie
Gevorderde omgewingsbeheertegnologie
Aanhangsel A: Metodes vir die bepaling van relatiewe molekulêre massaverspreiding van peptiede
Aanvaarding van standaard: GB/T 22492-2008
1 Toetsbeginsel:
Dit is bepaal deur hoëprestasie-gelfiltrasiechromatografie. Dit wil sê, deur poreuse vulstof as stasionêre fase te gebruik, gebaseer op die verskil in die relatiewe molekulêre massagrootte van die monsterkomponente vir skeiding, opgespoor by die peptiedbinding van die ultraviolet-absorpsiegolflengte van 220 nm, met behulp van die toegewyde dataverwerkingsagteware vir die bepaling van relatiewe molekulêre massaverspreiding deur gelfiltrasiechromatografie (d.w.s. die GPC-sagteware), is die chromatogramme en hul data verwerk, bereken om die grootte van die relatiewe molekulêre massa van die sojaboonpeptied en die verspreidingsreeks te verkry.
2. Reagense
Die eksperimentele water moet voldoen aan die spesifikasie van sekondêre water in GB/T6682, die gebruik van reagense, behalwe vir spesiale bepalings, is analities suiwer.
2.1 Reagense sluit in asetonitriel (chromatografies suiwer), trifluoroasynsuur (chromatografies suiwer),
2.2 Standaardstowwe wat in die kalibrasiekurwe van relatiewe molekulêre massaverspreiding gebruik word: insulien, mikopeptiede, glisien-glisien-tirosien-arginien, glisien-glisien-glisien
3 Instrument en toerusting
3.1 Hoëprestasievloeistofchromatograaf (HPLC): 'n chromatografiese werkstasie of integrator met 'n UV-detektor en GPC-dataverwerkingsagteware.
3.2 Mobiele fase vakuumfiltrasie- en ontgassingseenheid.
3.3 Elektroniese balans: gegradeerde waarde 0.000 1g.
4 Bedieningsstappe
4.1 Chromatografiese toestande en stelselaanpassingseksperimente (verwysingstoestande)
- 4.1.1 Chromatografiese kolom: TSKgelG2000swxl300 mm×7.8 mm (binnediameter) of ander gelkolomme van dieselfde tipe met soortgelyke werkverrigting geskik vir die bepaling van proteïene en peptiede.
- 4.1.2 Mobiele fase: Asetonitriel + water + trifluoroasynsuur = 20 + 80 + 0.1.
- 4.1.3 Deteksiegolflengte: 220 nm.
- 4.1.4 Vloeitempo: 0.5 ml/min.
- 4.1.5 Opsporingstyd: 30 min.
- 4.1.6 Monsterinspuitvolume: 20 μL.
- 4.1.7 Kolomtemperatuur: kamertemperatuur.
- 4.1.8 Om die chromatografiese stelsel aan die opsporingsvereistes te laat voldoen, is bepaal dat die doeltreffendheid van die gelchromatografiese kolom, d.w.s. die teoretiese aantal plate (N), onder die bogenoemde chromatografiese toestande nie minder as 10000 moet wees nie, bereken op grond van die pieke van die tripeptiedstandaard (Glisien-Glisien-Glisien).
- 4.2 Produksie van relatiewe molekulêre massa standaardkrommes
- Die bogenoemde verskillende relatiewe molekulêre massa peptiedstandaardoplossings met 'n massakonsentrasie van 1 mg/ml is voorberei deur mobiele fase-ooreenstemming, gemeng in 'n sekere verhouding, en dan gefiltreer deur 'n organiese fasemembraan met die poriegrootte van 0.2 μm~0.5 μm en ingespuit in die monster, en dan is die chromatogramme van die standaarde verkry. Relatiewe molekulêre massa-kalibrasiekrommes en hul vergelykings is verkry deur die logaritme van relatiewe molekulêre massa teen retensietyd of deur lineêre regressie te plot.
4.3 Monsterbehandeling
Weeg akkuraat 10 mg van die monster in 'n 10 ml maatfles, voeg 'n bietjie mobiele fase by, skud ultraklank vir 10 minute, sodat die monster volledig opgelos en gemeng is, verdun met mobiele fase tot op die skaal, en dan gefiltreer deur 'n organiese fasemembraan met 'n poriegrootte van 0.2 μm ~ 0.5 μm, en die filtraat is geanaliseer volgens die chromatografiese toestande in A.4.1.
- 5. Berekening van relatiewe molekulêre massaverspreiding
- Nadat die monsteroplossing wat in 4.3 voorberei is onder die chromatografiese toestande van 4.1 geanaliseer is, kan die relatiewe molekulêre massa van die monster en die verspreidingsreeks daarvan verkry word deur die chromatografiese data van die monster in die kalibrasiekurwe 4.2 met GPC-dataverwerkingsagteware in te voeg. Die verspreiding van die relatiewe molekulêre massas van die verskillende peptiede kan bereken word deur die piekarea-normaliseringsmetode, volgens die formule: X=A/A totaal×100
- In die formule: X - Die massafraksie van 'n relatiewe molekulêre massa peptied in die totale peptied in die monster, %;
- A - Piekarea van 'n relatiewe molekulêre massa peptied;
- Totaal A - die som van die piekoppervlaktes van elke relatiewe molekulêre massa peptied, bereken tot een desimale plek.
- 6 Herhaalbaarheid
- Die absolute verskil tussen twee onafhanklike bepalings wat onder herhaalbaarheidstoestande verkry is, mag nie 15% van die rekenkundige gemiddelde van die twee bepalings oorskry nie.
- Aanhangsel B: Metodes vir die bepaling van vrye aminosure
- Aanvaarding van standaard: Q/320205 KAVN05-2016
- 1.2 Reagense en materiale
- Ysasynsuur: analities suiwer
- Perchloorsuur: 0.0500 mol/L
- Aanwyser: 0.1% kristalviolet-indikator (ysasynsuur)
- 2. Bepaling van vrye aminosure
Die monsters is vir 1 uur by 80°C gedroog.
Plaas die monster in 'n droë houer om natuurlik af te koel tot kamertemperatuur of af te koel tot 'n bruikbare temperatuur.Weeg ongeveer 0.1 g van die monster (akkuraat tot 0.001 g) in 'n 250 mL droë koniese fles.Gaan vinnig voort na die volgende stap om te verhoed dat die monster omgewingsvog absorbeer.Voeg 25 ml ysasynsuur by en meng goed vir nie meer as 5 minute nie.Voeg 2 druppels kristalvioletindikator byTitreer met 0.0500 mol / L (±0.001) standaard titrasie-oplossing van perchloorsuur totdat die oplossing van pers na die eindpunt verander.
Teken die volume van die standaardoplossing wat verbruik is aan.
- Voer die blankotoets terselfdertyd uit.
- 3. Berekening en resultate
- Die vrye aminosuurinhoud X in die reagens word uitgedruk as 'n massafraksie (%) en word bereken volgens die formule: X = C × (V1-V0) × 0.1445/M × 100%, in die formule:
- C - Konsentrasie van standaard perchloorsuuroplossing in mol per liter (mol/L)
- V1 - Volume gebruik vir titrasie van monsters met standaard perchloorsuuroplossing, in milliliter (mL).
- Vo - Volume gebruik vir titrasie-blanko met standaard perchloorsuuroplossing, in milliliter (mL);
M - Massa van die monster, in gram (g).
| 0.1445: Gemiddelde massa aminosure gelykstaande aan 1.00 mL standaard perchloorsuuroplossing [c (HClO4) = 1.000 mol / L]. | 4.2.3 Seriumsulfaat standaard titrasie-oplossing: konsentrasie c [Ce (SO4) 2] = 0.1 mol/L, voorberei volgens GB/T601. | |
| Aanvaarding van standaarde: Q/70920556 71-2024 | 1. Bepalingsbeginsel (Fe as voorbeeld) | Aminosuur-ysterkomplekse het baie lae oplosbaarheid in anhidriese etanol en vrymetaalione is oplosbaar in anhidriese etanol. Die verskil in oplosbaarheid tussen die twee in anhidriese etanol is gebruik om die chelasietempo van aminosuur-ysterkomplekse te bepaal. |
| In die formule: V1 - volume van seriumsulfaatstandaardoplossing verbruik vir titrasie van toetsoplossing, mL; | Watervrye etanol; die res is dieselfde as klousule 4.5.2 in GB/T 27983-2011. | 3. Stappe van analise |
| Doen twee proewe parallel. Weeg 0.1 g van die monster, gedroog by 103 ± 2 ℃ vir 1 uur, akkuraat tot 0.0001 g, voeg 100 ml anhidriese etanol by om op te los, filtreer, filtreer die residu en was dit ten minste drie keer met 100 ml anhidriese etanol. Dra dan die residu oor na 'n 250 ml koniese fles, voeg 10 ml swaelsuuroplossing by volgens klousule 4.5.3 in GB/T27983-2011, en voer dan die volgende stappe uit volgens klousule 4.5.3 "Verhit om op te los en laat dan afkoel" in GB/T27983-2011. Voer die blankotoets terselfdertyd uit. | 4. Bepaling van totale ysterinhoud | 4.1 Die bepalingsbeginsel is dieselfde as klousule 4.4.1 in GB/T 21996-2008. |
4.2. Reagense en oplossings
| 4.2.1 Gemengde suur: Voeg 150 ml swaelsuur en 150 ml fosforsuur by 700 ml water en meng goed. | 4.2.2 Natriumdifenielamiensulfonaat-indikatoroplossing: 5g/L, voorberei volgens GB/T603. | 4.2.3 Seriumsulfaat standaard titrasie-oplossing: konsentrasie c [Ce (SO4) 2] = 0.1 mol/L, voorberei volgens GB/T601. | |
| 4.3 Stappe van analise | Doen twee proewe parallel. Weeg 0.1 g monster, akkuraat tot 020001 g, plaas in 'n 250 ml koniese fles, voeg 10 ml gemengde suur by, voeg na oplossing 30 ml water en 4 druppels natriumdianiliensulfonaat-indikatoroplossing by, en voer dan die volgende stappe uit volgens klousule 4.4.2 in GB/T21996-2008. Voer die blankotoets terselfdertyd uit. | 4.4 Voorstelling van resultate | Die totale ysterinhoud X1 van die aminosuur-ysterkomplekse in terme van massafraksie van yster, die waarde uitgedruk in %, is bereken volgens formule (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - seriumsulfaat standaardoplossing verbruik vir titrasie van blanko oplossing, mL; | V0 - seriumsulfaat standaardoplossing verbruik vir titrasie van blanko oplossing, mL; | C - Werklike konsentrasie van seriumsulfaat standaardoplossing, mol/L5. Berekening van ysterinhoud in chelateDie ysterinhoud X2 in die chelaat in terme van die massafraksie van yster, die waarde uitgedruk in %, is bereken volgens die formule: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| In die formule: V1 - volume van seriumsulfaatstandaardoplossing verbruik vir titrasie van toetsoplossing, mL; | V2 - seriumsulfaat standaardoplossing verbruik vir titrasie van blanko oplossing, mL;nom1-Massa van die monster, g. Neem die rekenkundige gemiddelde van die parallelle bepalingsresultate as die bepalingsresultate, en die absolute verskil van die parallelle bepalingsresultate is nie meer as 0.3% nie. | 0.05585 - massa ysterhoudende yster uitgedruk in gram gelykstaande aan 1.00 mL seriumsulfaat standaardoplossing C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Massa van die monster, g. Neem die rekenkundige gemiddelde van die parallelle bepalingsresultate as die bepalingsresultate, en die absolute verskil van die parallelle bepalingsresultate is nie meer as 0.3% nie. | 6. Berekening van chelasietempoChelaeringskoers X3, die waarde uitgedruk in %, X3 = X2/X1 × 100Aanhangsel C: Metodes vir die bepaling van Zinpro se chelasietempo |
Aanvaarding van standaard: Q/320205 KAVNO7-2016
1. Reagense en materiale
a) Ysasynsuur: analities suiwer; b) Perchloorsuur: 0.0500mol/L; c) Indikator: 0.1% kristalvioletindikator (ysasynsuur)
2. Bepaling van vrye aminosure
2.1 Die monsters is vir 1 uur by 80°C gedroog.
2.2 Plaas die monster in 'n droë houer om natuurlik af te koel tot kamertemperatuur of af te koel tot 'n bruikbare temperatuur.
2.3 Weeg ongeveer 0.1 g monster (akkuraat tot 0.001 g) in 'n 250 mL droë koniese fles.
2.4 Gaan vinnig voort na die volgende stap om te verhoed dat die monster omgewingsvog absorbeer.
2.5 Voeg 25 ml ysasynsuur by en meng goed vir nie meer as 5 minute nie.
2.6 Voeg 2 druppels kristalvioletindikator by.
2.7 Titreer met 0.0500mol/L (±0.001) standaard titrasie-oplossing van perchloorsuur totdat die oplossing vir 15 sekondes van pers na groen verander sonder om van kleur as die eindpunt te verander.
2.8 Teken die volume van die standaardoplossing wat verbruik is aan.
2.9 Voer die blankotoets terselfdertyd uit.
- 3. Berekening en resultate
- Katalaans
- Physicochemical parameters
V1 - Volume gebruik vir titrasie van monsters met standaard perchloorsuuroplossing, in milliliter (mL).
Vo - Volume gebruik vir titrasie-blanko met standaard perchloorsuuroplossing, in milliliter (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Adres: No.147 Qingpu-pad, Shouan-stad, Pujiang-distrik, Chengdu-stad, Sichuan-provinsie, China
Foon: 86-18880477902
Produkte
Anorganiese spoorminerale
- Organiese spoorminerale
- Swahili
- Gepasmaakte diens
- Vinnige skakels
Maatskappyprofiel
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Goedjarati | Klik vir navraag | © Kopiereg - 2010-2025: Alle regte voorbehou. | Werfkaart TOP SOEK Foon |
| Tel. | 86-18880477902 | Javaans | E-pos |
| 8618880477902 | Chinees | Frans | |
| Bird | Chinees | Frans | Duits Spaans |
| Aquatic animals | Japannees | Koreaans | Arabies Grieks |
| Turks | Italiaans | ||
| Ruminant animal g/head day | January 0.75 | Indonesies Afrikaans Sweeds |
Pools
- Baskies
- Katalaans
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Sjona
Bulgaars
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroaties
Nederlands
| Application object | Oerdoe Viëtnamees | Content in full-value feed (mg/kg) | Efficacy |
| Goedjarati | Haïtiaans | Hausa | Kinyarwanda Hmong Hongaars |
| Piglets and fattening pigs | Igbo | Javaans | Kannada Khmer Koerdies |
| Kirgisies | Latyn | ||
| Bird | 300～400 | 45～60 | Masedonies Maleis Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Noors
- Pasjtoe
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serwies
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Sjona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili
Tadjik
Tamil
Telugu
Thai
| Application object | Oerdoe Viëtnamees | Content in full-value feed (mg/kg) | Efficacy |
| Jiddisj | Yoruba | Zoeloe | Kinyarwanda Oriya Turkmeens |
| Oeigoer | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
